A Flow-Cytometry-Based Approach to Facilitate Quantification, Size Estimation and Characterization of Sub-visible Particles in Protein Solutions

Christian Lubich; Mantas Malisauskas; Thomas Prenninger; Thomas Wurz; Peter Matthiessen; Peter L. Turecek; Friedrich Scheiflinger; Birgit M. Reipert

doi:10.1007/s11095-015-1669-3

A Flow-Cytometry-Based Approach to Facilitate Quantification, Size Estimation and Characterization of Sub-visible Particles in Protein Solutions

Christian Lubich, Mantas Malisauskas, Thomas Prenninger, Thomas Wurz, Peter Matthiessen, Peter L. Turecek, Friedrich Scheiflinger, Birgit M. Reipert

Baxter Innovation GmbH

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

Abstract

Sub-visible particles were shown to facilitate unwanted immunogenicity of protein therapeutics. To understand the root cause of this phenomenon, a comprehensive analysis of these particles is required. We aimed at establishing a flow-cytometry-based technology to analyze the amount, size distribution and nature of sub-visible particles in protein solutions.

Originalsprache	Englisch
Aufsatznummer	1669
Seiten (von - bis)	2863-2876
Seitenumfang	14
Fachzeitschrift	Pharmaceutical Research
Jahrgang	32
Ausgabenummer	9
DOIs	https://doi.org/10.1007/s11095-015-1669-3
Publikationsstatus	Veröffentlicht - 19 März 2015
Extern publiziert	Ja

ÖFOS 2012 - Österreichischen Systematik der Wissenschaftszweige

304005 Medizinische Biotechnologie

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10.1007/s11095-015-1669-3

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Lubich, C., Malisauskas, M., Prenninger, T., Wurz, T., Matthiessen, P., Turecek, P. L., Scheiflinger, F., & Reipert, B. M. (2015). A Flow-Cytometry-Based Approach to Facilitate Quantification, Size Estimation and Characterization of Sub-visible Particles in Protein Solutions. Pharmaceutical Research, 32(9), 2863-2876. Artikel 1669. https://doi.org/10.1007/s11095-015-1669-3

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