TY - JOUR
T1 - A Flow-Cytometry-Based Approach to Facilitate Quantification, Size Estimation and Characterization of Sub-visible Particles in Protein Solutions
AU - Lubich, Christian
AU - Malisauskas, Mantas
AU - Prenninger, Thomas
AU - Wurz, Thomas
AU - Matthiessen, Peter
AU - Turecek, Peter L.
AU - Scheiflinger, Friedrich
AU - Reipert, Birgit M.
N1 - Publisher Copyright:
© 2015 Springer Science+Business Media New York.
PY - 2015/3/19
Y1 - 2015/3/19
N2 - Sub-visible particles were shown to facilitate unwanted immunogenicity of protein therapeutics. To understand the root cause of this phenomenon, a comprehensive analysis of these particles is required. We aimed at establishing a flow-cytometry-based technology to analyze the amount, size distribution and nature of sub-visible particles in protein solutions.
AB - Sub-visible particles were shown to facilitate unwanted immunogenicity of protein therapeutics. To understand the root cause of this phenomenon, a comprehensive analysis of these particles is required. We aimed at establishing a flow-cytometry-based technology to analyze the amount, size distribution and nature of sub-visible particles in protein solutions.
KW - cross-β-sheet structures
KW - flow cytometry
KW - protein aggregates
KW - protein therapeutics
KW - sub-visible particles
UR - http://www.scopus.com/inward/record.url?scp=84938747649&partnerID=8YFLogxK
U2 - 10.1007/s11095-015-1669-3
DO - 10.1007/s11095-015-1669-3
M3 - Article
SN - 1573-904X
VL - 32
SP - 2863
EP - 2876
JO - Pharmaceutical Research
JF - Pharmaceutical Research
IS - 9
M1 - 1669
ER -