@article{a763321589a94c4fa8cff85caede1952,
title = "A Screening Approach for Identifying Gliadin Neutralizing Antibodies on Epithelial Intestinal Caco-2 Cells: SLAS Discovery",
abstract = "Celiac disease (CD) is a chronic inflammatory condition caused by the ingestion of gliadin-containing food in genetically susceptible individuals. Undigested peptides of gliadin exert various effects, including increased intestinal permeability and inflammation in the small intestine. Although many therapeutic approaches are in development, a gluten-free diet is the only effective treatment for CD. Affecting at least 1% of the population in industrialized countries, it is important to generate therapeutic options against CD. Here, we describe the establishment of a high-throughput screening (HTS) platform based on AlphaLISA and electrical cell–substrate impedance sensing (ECIS) technology for the identification of anti-inflammatory and barrier-protective compounds in human enterocytes after pepsin-trypsin-digested gliadin (PT-gliadin) treatment. Our results show that the combination of these HTS technologies enables fast, reliable, simple, and label-free screening of IgY antibodies against PT-gliadin. Using this platform, we have identified a new chicken anti-PT-gliadin IgY antibody as a potential anti-CD agent.",
keywords = "gliadin antibody, immunoglobulin Y antibody, interleukin 8, neutralizing antibody, recombinant tumor necrosis factor, transcription factor RelA, unclassified drug, gliadin, immunoglobulin, immunoglobulin enhancer binding protein, immunoglobulin Y, transcription factor, antibody screening, antibody specificity, Article, binding affinity, Caco-2 cell line, cell function, cell membrane permeability, cell survival, cell viability assay, controlled study, down regulation, electric cell substrate impedance sensing, enzyme linked immunosorbent assay, high throughput screening, human, human cell, impedance, intestine epithelium, permeability barrier, protein expression, protein phosphorylation, Western blotting, cell communication, cytology, epithelium cell, immunology, inflammation, intestine, isolation and purification, metabolism, pathology, procedures, Antibodies, Neutralizing, Caco-2 Cells, Cell Communication, Cell Survival, Down-Regulation, Epithelial Cells, Gliadin, High-Throughput Screening Assays, Humans, Immunoglobulins, Inflammation, Interleukin-8, Intestines, NF-kappa B, Transcription Factors, AlphaLISA, Caco-2 monolayer, celiac disease, anti-gliadin IgY, ECIS",
author = "Harald Hundsberger and Anita Koppensteiner and Elisabeth Hofmann and Doris Ripper and Maren Pfl{\"u}ger and Valerie Stadlmann and Klein, {Christian Theodor} and B. Kreiseder and M. Katzlinger and Andreas Eger and Florian Forster and A. Missbichler and Christoph Wiesner",
note = "Funding Information: The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Austrian FFG (Forschungs F{\"o}rderungs Gesellschaft) Project COIN, Nr. 286415 to Christoph Wiesner and Project BRIDGE, Nr. 2647553 to Harald Hundsberger. Publisher Copyright: {\textcopyright} 2017, {\textcopyright} 2017 Society for Laboratory Automation and Screening.",
year = "2017",
month = sep,
day = "8",
doi = "10.1177/2472555217697435",
language = "English",
volume = "22",
pages = "1035--1043",
journal = "SLAS Discovery",
issn = "2472-5552",
publisher = "Society for Laboratory Automation and Screening",
number = "8",
}
