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Abstract
The interaction between monocytes and endothelial cells in inflammation is central to chemoattraction, adhesion, and transendothelial migration. Key players, such as selectins and their ligands, integrins, and other adhesion molecules, and their functions in these processes are well studied. Toll-like receptor 2 (TLR2), expressed in monocytes, is critical for sensing invading pathogens and initiating a rapid and effective immune response. However, the extended role of TLR2 in monocyte adhesion and migration has only been partially elucidated. To address this question, we performed several functional cell-based assays using monocyte-like wild type (WT), TLR2 knock-out (KO), and TLR2 knock-in (KI) THP-1 cells. We found that TLR2 promotes the faster and stronger adhesion of monocytes to the endothelium and a more intense endothelial barrier disruption after endothelial activation. In addition, we performed quantitative mass spectrometry, STRING protein analysis, and RT-qPCR, which not only revealed the association of TLR2 with specific integrins but also uncovered novel proteins affected by TLR2. In conclusion, we show that unstimulated TLR2 influences cell adhesion, endothelial barrier disruption, migration, and actin polymerization.
Originalsprache | Englisch |
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Aufsatznummer | 1425 |
Fachzeitschrift | Cells |
Jahrgang | 12 |
Ausgabenummer | 10 |
DOIs | |
Publikationsstatus | Veröffentlicht - 19 Mai 2023 |
IMC Forschungsschwerpunkte
- Medical biotechnology
ÖFOS 2012 - Österreichischen Systematik der Wissenschaftszweige
- 304005 Medizinische Biotechnologie
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Entwicklung leistungsfähiger Diagnostikverfahren und neuer Therapieansätze in Inflammation und Sepsis
Wiesner, C. (Leitende(r) Forscher/-in), Grünstäudl, P. (Weitere Forschende), Colleselli, K. (Weitere Forschende) & Stierschneider, A. (Weitere Forschende)
18/09/19 → 24/01/24
Projekt: Forschung