C-Terminal Motifs of the MTW1 Complex Cooperatively Stabilize Outer Kinetochore Assembly in Budding Yeast

Medini Ghodgaonkar-Steger, Mia Potocnjak, Tomasz Zimniak, Josef Fischböck-Halwachs, Victor Solis-Mezarino, Sylvia Singh, Tea Speljko, Götz Hagemann, David Jan Drexler, Gregor Witte, Franz Herzog

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

Kinetochores are macromolecular protein assemblies at centromeres that mediate accurate chromosome segregation during cell division. The outer kinetochore KNL1 SPC105, MIS12 MTW1, and NDC80 NDC80 complexes assemble the KMN network, which harbors the sites of microtubule binding and spindle assembly checkpoint signaling. The buildup of the KMN network that transmits microtubule pulling forces to budding yeast point centromeres is poorly understood. Here, we identify 225 inter-protein crosslinks by mass spectrometry on KMN complexes isolated from Saccharomyces cerevisiae that delineate the KMN subunit connectivity for outer kinetochore assembly. C-Terminal motifs of Nsl1 and Mtw1 recruit the SPC105 complex through Kre28, and both motifs aid tethering of the NDC80 complex by the previously reported Dsn1 C terminus. We show that a hub of three C-terminal MTW1 subunit motifs mediates the cooperative stabilization of the KMN network, which is augmented by a direct NDC80-SPC105 association.

OriginalspracheEnglisch
Aufsatznummer108190
Seiten (von - bis)108190
FachzeitschriftCell Reports
Jahrgang32
Ausgabenummer13
DOIs
PublikationsstatusVeröffentlicht - 29 Sep. 2020
Extern publiziertJa

Fingerprint

Untersuchen Sie die Forschungsthemen von „C-Terminal Motifs of the MTW1 Complex Cooperatively Stabilize Outer Kinetochore Assembly in Budding Yeast“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren