Characterization of Natronobacterium magadii phage ΦGh1, a unique archaeal phage containing DNA and RNA

A. Witte, U. Baranyi, R. Klein, M. Sulzner, C. Luo, G. Wanner, D.H. Krüger, W. Lubitz

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

A novel archaeal bacteriophage, ΦCh1, was isolated from a haloalkalophilic archaeon Natronobacterium magadii upon spontaneous lysis. The phage-cured strain N. magadii(L13) was used to demonstrate infectivity of phage ΦCh1. The turbid-plaque morphology and the fact that N. magadii cells isolated from plaques were able to produce phage indicated that ΦCh1 is a temperate phage. The phage morphology resembles other members of Myoviridae-infecting Halobacterium species. In solution below 2M NaCl, the phage lost its morphological stability and infectivity. One- and two-dimensional SDS-PAGE of phage particles revealed at least four major and five minor proteins with molecular masses ranging from 15 to 80kDa and acidic isoelectric points. Southern blot analysis of chromosomal DNA of a lysogenic N. magadii strain showed that ΦCh1 exists as a chromosomally integrated prophage. The phage particles contain both double-stranded, linear DNA (approx. 55kbp) as well as several RNA species (80-700 nucleotides). Hybridization of labelled RNA fragments to total DNA from N. magadii and ΦCh1 showed that the virion-associated RNA is host encoded. Part of the phage DNA population is modified and restriction analysis revealed evidence for adenine methylation. Phage ΦCh1 is the first virus described for the genus Natronobacterium, and the first phage containing DNA and RNA in mature phage particles.
OriginalspracheEnglisch
Seiten (von - bis)603-616
Seitenumfang14
FachzeitschriftMolecular Microbiology
Jahrgang23
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - 31 Okt. 2003
Extern publiziertJa

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