Chiral Hydroxylation at the Mononuclear Nonheme Fe(II) Center of 4-(S) Hydroxymandelate Synthase - A Structure-Activity Relationship Analysis

C.M.L. Di Giuro, C. Konstantinovics, U. Rinner, C. Nowikow, E. Leitner, G.D. Straganz

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

(S)-Hydroxymandelate synthase (Hms) is a nonheme Fe(II) dependent dioxygenase that catalyzes the oxidation of 4-hydroxyphenylpyruvate to (S)-4-hydroxymandelate by molecular oxygen. In this work, the substrate promiscuity of Hms is characterized in order to assess its potential for the biosynthesis of chiral α-hydroxy acids. Enzyme kinetic analyses, the characterization of product spectra, quantitative structure activity relationship (QSAR) analyses and in silico docking studies are used to characterize the impact of substrate properties on particular steps of catalysis. Hms is found to accept a range of α-oxo acids, whereby the presence of an aromatic substituent is crucial for efficient substrate turnover. A hydrophobic substrate binding pocket is identified as the likely determinant of substrate specificity. Upon introduction of a steric barrier, which is suspected to obstruct the accommodation of the aromatic ring in the hydrophobic pocket during the final hydroxylation step, the racemization of product is obtained. A steady state kinetic analysis reveals that the turnover number of Hms strongly correlates with substrate hydrophobicity. The analysis of product spectra demonstrates high regioselectivity of oxygenation and a strong coupling efficiency of C-C bond cleavage and subsequent hydroxylation for the tested substrates. Based on these findings the structural basis of enantioselectivity and enzymatic activity is discussed.

OriginalspracheEnglisch
Aufsatznummere68932
Seiten (von - bis)e68932
FachzeitschriftPLoS ONE
Jahrgang8
Ausgabenummer7
DOIs
PublikationsstatusVeröffentlicht - 23 Juli 2013
Extern publiziertJa

Forschungsfelder

  • Struktur-Wirkungs-Beziehung

IMC Forschungsschwerpunkte

  • Materials science

ÖFOS 2012 - Österreichischen Systematik der Wissenschaftszweige

  • 301305 Medizinische Chemie
  • 209001 Biokatalyse

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