TY - JOUR
T1 - Determination of the minimum fully protective dose of adenovirus-based DIVA vaccine against peste des petits ruminants virus challenge in East African goats
AU - Holzer, Barbara
AU - Taylor, Geraldine
AU - Rajko-Nenow, Paulina
AU - Hodgson, Sophia
AU - Okoth, Edward
AU - Herbert, Rebecca
AU - Toye, Philip
AU - Baron, Michael D
N1 - Publisher Copyright:
© 2016 Holzer et al.
PY - 2016/1/21
Y1 - 2016/1/21
N2 - Peste des petits ruminants virus (PPRV) causes an economically important disease of sheep and goats, primarily in developing countries. It is becoming the object of intensive international control efforts. Current vaccines do not allow vaccinated and infected animals to be distinguished (no DIVA capability). We have previously shown that recombinant, replication-defective, adenovirus expressing the PPRV H glycoprotein (AdH) gives full protection against wild type PPRV challenge. We have now tested lower doses of the vaccine, as well as AdH in combination with a similar construct expressing the PPRV F glycoprotein (AdF). We show here that, in a local breed of goat in a country where PPR disease is common (Kenya), as little as 10(7) pfu of AdH gives significant protection against PPRV challenge, while a vaccine consisting of 10(8) pfu of each of AdH and AdF gives apparently sterile protection. These findings underline the utility of these constructs as DIVA vaccines for use in PPR control.
AB - Peste des petits ruminants virus (PPRV) causes an economically important disease of sheep and goats, primarily in developing countries. It is becoming the object of intensive international control efforts. Current vaccines do not allow vaccinated and infected animals to be distinguished (no DIVA capability). We have previously shown that recombinant, replication-defective, adenovirus expressing the PPRV H glycoprotein (AdH) gives full protection against wild type PPRV challenge. We have now tested lower doses of the vaccine, as well as AdH in combination with a similar construct expressing the PPRV F glycoprotein (AdF). We show here that, in a local breed of goat in a country where PPR disease is common (Kenya), as little as 10(7) pfu of AdH gives significant protection against PPRV challenge, while a vaccine consisting of 10(8) pfu of each of AdH and AdF gives apparently sterile protection. These findings underline the utility of these constructs as DIVA vaccines for use in PPR control.
UR - http://www.scopus.com/inward/record.url?scp=84954517708&partnerID=8YFLogxK
U2 - 10.1186/s13567-016-0306-4
DO - 10.1186/s13567-016-0306-4
M3 - Article
SN - 0928-4249
VL - 47
SP - 20
JO - Veterinary Research
JF - Veterinary Research
IS - 1
M1 - 20
ER -