Establishment of a Pig Influenza Challenge Model for Evaluation of Monoclonal Antibody Delivery Platforms

Adam McNee, Trevor R F Smith, Barbara Holzer, Becky Clark, Emily Bessell, Ghiabe Guibinga, Heather Brown, Katherine Schultheis, Paul Fisher, Stephanie Ramos, Alejandro Nunez, Matthieu Bernard, Simon Graham, Veronica Martini, Tiphany Chrun, Yongli Xiao, John C Kash, Jeffery K Taubenberger, Sarah Elliott, Ami PatelPeter Beverley, Pramila Rijal, David B Weiner, Alain Townsend, Kate E Broderick, Elma Tchilian

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung


mAbs are a possible adjunct to vaccination and drugs in treatment of influenza virus infection. However, questions remain whether small animal models accurately predict efficacy in humans. We have established the pig, a large natural host animal for influenza, with many physiological similarities to humans, as a robust model for testing mAbs. We show that a strongly neutralizing mAb (2-12C) against the hemagglutinin head administered prophylactically at 15 mg/kg reduced viral load and lung pathology after pandemic H1N1 influenza challenge. A lower dose of 1 mg/kg of 2-12C or a DNA plasmid-encoded version of 2-12C reduced pathology and viral load in the lungs but not viral shedding in nasal swabs. We propose that the pig influenza model will be useful for testing candidate mAbs and emerging delivery platforms prior to human trials.
Seiten (von - bis)648—660
FachzeitschriftJournal of Immunology
PublikationsstatusVeröffentlicht - 1 Aug. 2020
Extern publiziertJa

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