@article{6528c5dce7be4854b677cfb14141f108,
title = "Novel aeruginosin-865 from Nostoc sp. as a potent anti-inflammatory agent",
abstract = "Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Luke{\v s}ov{\'a} 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-α-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC 50 values of (3.5±1.5) μg mL -1 ((4.0±1.7) μM) and (50.0±13.4) μg mL -1 ((57.8±15.5) μM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-κB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect.",
keywords = "aeruginosin 865, antiinflammatory agent, bacterial protein, intercellular adhesion molecule 1, interleukin 8, peptide, unclassified drug, amino acid sequence, antiinflammatory activity, article, carbon nuclear magnetic resonance, cell adhesion, cell survival, cell viability, chiral chromatography, confocal laser microscopy, controlled study, down regulation, extraction, heteronuclear multiple bond correlation, heteronuclear single quantum coherence, high performance liquid chromatography, human, human cell, mass spectrometry, microvascular endothelial cell, Nostoc, nuclear localization signal, nuclear magnetic resonance spectroscopy, priority journal, purification, stereochemistry, Cyanobacteria, Nostoc sp., anti-inflammatory, cyanobacteria, drug discovery, NMR spectroscopy, peptides, Active Transport, Cell Nucleus, Anti-Inflammatory Agents, Non-Steroidal, Cell Line, Dose-Response Relationship, Drug, Down-Regulation, Glycopeptides, Humans, Intercellular Adhesion Molecule-1, Interleukin-8, Lipopeptides, Molecular Structure, NF-kappa B, Protein Transport, Structure-Activity Relationship",
author = "A. Kapu{\'s}cik and P. Hrouzek and M. Kuzma and S. B{\'a}rtov{\'a} and P. Nov{\'a}k and J. Jokela and M. Pfl{\"u}ger and A. Eger and H. Hundsberger and J. Kopeck{\'y}",
note = "cited By 30",
year = "2013",
month = oct,
day = "2",
doi = "10.1002/cbic.201300246",
language = "English",
volume = "14",
pages = "2329--2337",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "17",
}
