Novel aeruginosin-865 from Nostoc sp. as a potent anti-inflammatory agent

A. Kapuścik, P. Hrouzek, M. Kuzma, S. Bártová, P. Novák, J. Jokela, M. Pflüger, A. Eger, H. Hundsberger, J. Kopecký

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung


Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukešová 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-α-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC 50 values of (3.5±1.5) μg mL -1 ((4.0±1.7) μM) and (50.0±13.4) μg mL -1 ((57.8±15.5) μM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-κB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect.

Seiten (von - bis)2329-2337
PublikationsstatusVeröffentlicht - 2 Okt. 2013


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