Probing native protein structures by chemical cross-linking, mass spectrometry, and bioinformatics

Alexander Leitner, Thomas Walzthoeni, Abdullah Kahraman, Franz Herzog, Oliver Rinner, Martin Beck, Ruedi Aebersold

Publikation: Beitrag in FachzeitschriftÜbersichtsartikelBegutachtung

Abstract

Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of cross-linking data for computational modeling. Finally, the impact of protein cross-linking on various biological disciplines is highlighted.

OriginalspracheEnglisch
Seiten (von - bis)1634-1649
Seitenumfang16
FachzeitschriftMolecular and Cellular Proteomics
Jahrgang9
Ausgabenummer8
DOIs
PublikationsstatusVeröffentlicht - Aug. 2010
Extern publiziertJa

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