Single cell sequencing identifies clonally expanded synovial CD4 + T PH cells expressing GPR56 in rheumatoid arthritis.

Alexandra Argyriou, Marc H Wadsworth, Adrian Lendvai, Stephen M Christensen, Aase H Hensvold, Christina Gerstner, Annika van Vollenhoven, Kellie Kravarik, Aaron Winkler, Vivianne Malmström, Karine Chemin

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease affecting synovial joints where different CD4 + T cell subsets may contribute to pathology. Here, we perform single cell sequencing on synovial CD4 + T cells from anti-citrullinated protein antibodies (ACPA)+ and ACPA- RA patients and identify two peripheral helper T cell (T PH) states and a cytotoxic CD4 + T cell subset. We show that the adhesion G-protein coupled receptor 56 (GPR56) delineates synovial CXCL13 high T PH CD4 + T cells expressing LAG-3 and the tissue-resident memory receptors CXCR6 and CD69. In ACPA- SF, T PH cells display lower levels of GPR56 and LAG-3. Further, most expanded T cell clones in the joint are within CXCL13 high T PH CD4 + T cells. Finally, RNA-velocity analyses suggest a common differentiation pathway between the two T PH clusters and effector CD4 + T cells. Our study provides comprehensive immunoprofiling of the synovial CD4 + T cell subsets in ACPA+ and ACPA- RA.

OriginalspracheEnglisch
Aufsatznummer4046
Seiten (von - bis)4046
FachzeitschriftNature Communications
Jahrgang13
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - 13 Juli 2022
Extern publiziertJa

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