Structural and functional insights into the fly microRNA biogenesis factor Loquacious

Leonhard Jakob, Thomas Treiber, Nora Treiber, Alexander Gust, Kevin Kramm, Kerrin Hansen, Mathias Stotz, Ludwig Wankerl, Franz Herzog, Stefan Hannus, Dina Grohmann, Gunter Meister

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

In the microRNA (miRNA) pathway, Dicer processes precursors to mature miRNAs. For efficient processing, double-stranded RNA-binding proteins support Dicer proteins. In flies, Loquacious (Loqs) interacts with Dicer1 (dmDcr1) to facilitate miRNA processing. Here, we have solved the structure of the third double-stranded RNA-binding domain (dsRBD) of Loqs and define specific structural elements that interact with dmDcr1. In addition, we show that the linker preceding dsRBD3 contributes significantly to dmDcr1 binding. Furthermore, our structural work demonstrates that the third dsRBD of Loqs forms homodimers. Mutations in the dimerization interface abrogate dmDcr1 interaction. Loqs, however, binds to dmDcr1 as a monomer using the identified dimerization surface, which suggests that Loqs might form dimers under conditions where dmDcr1 is absent or not accessible. Since critical sequence elements are conserved, we suggest that dimerization might be a general feature of dsRBD proteins in gene silencing.

OriginalspracheEnglisch
Seiten (von - bis)383-396
Seitenumfang14
FachzeitschriftRNA
Jahrgang22
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - März 2016
Extern publiziertJa

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