The COMA complex interacts with Cse4 and positions Sli15/ipl1 at the budding yeast inner kinetochore

Josef Fischböck-Halwachs, Sylvia Singh, Mia Potocnjak, Götz Hagemann, Victor Solis-Mezarino, Stephan Woike, Medini Ghodgaonkar-Steger, Florian Weissmann, Laura D. Gallego, Julie Rojas, Jessica Andreani, Alwin Köhler, Franz Herzog

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

Kinetochores are macromolecular protein complexes at centromeres that ensure accurate chromosome segregation by attaching chromosomes to spindle microtubules and integrating safeguard mechanisms. The inner kinetochore is assembled on CENP-A nucleosomes and has been implicated in establishing a kinetochore-associated pool of Aurora B kinase, a chromosomal passenger complex (CPC) subunit, which is essential for chromosome biorientation. By performing crosslink-guided in vitro reconstitution of budding yeast kinetochore complexes we showed that the Ame1/Okp1CENP-U/Q heterodimer, which forms the COMA complex with Ctf19/McM21CENP-P/O, selectively bound Cse4CENP-A nucleosomes through the Cse4 N-terminus. The Sli15/Ipl1INCENP/Aurora-B core-CPC interacted with COMA in vitro through the Ctf19 C-terminus whose deletion affected chromosome segregation fidelity in Sli15 wild-type cells. Tethering Sli15 to Ame1/Okp1 rescued synthetic lethality upon Ctf19 depletion in a Sli15 centromere-targeting deficient mutant. This study shows molecular characteristics of the point-centromere kinetochore architecture and suggests a role for the Ctf19 C-terminus in mediating CPC-binding and accurate chromosome segregation.

OriginalspracheEnglisch
Aufsatznummere42879
FachzeitschrifteLife
Jahrgang8
DOIs
PublikationsstatusVeröffentlicht - 21 Mai 2019
Extern publiziertJa

Forschungsfelder

  • Cell Division
  • Chemical Crosslinking
  • Mass spectrometry
  • Structural Proteomics

IMC Forschungsschwerpunkte

  • Medical biotechnology

ÖFOS 2012 - Österreichischen Systematik der Wissenschaftszweige

  • 106037 Proteomik
  • 106041 Strukturbiologie
  • 106044 Systembiologie

Fingerprint

Untersuchen Sie die Forschungsthemen von „The COMA complex interacts with Cse4 and positions Sli15/ipl1 at the budding yeast inner kinetochore“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren