TY - JOUR
T1 - The effect of lanthanides on photosynthesis, growth, and chlorophyll profile of the green alga Desmodesmus quadricauda
AU - Řezanka, T.
AU - Kaineder, K.
AU - Mezricky, D.
AU - Řezanka, M.
AU - Bišová, K.
AU - Zachleder, V.
AU - Vítová, M.
N1 - Funding Information:
This work was supported by the Project LO1201 and LO1416 obtained through financial support of the Ministry of Education, Youth and Sports in the targeted framework of the National Programme for Sustainability, by the Grant Agency of the Czech Republic P 503 14–00227S and Institutional Research Concept RVO61388971.
Publisher Copyright:
© 2016, Springer Science+Business Media Dordrecht.
PY - 2016/4/25
Y1 - 2016/4/25
N2 - Lanthanides (La, Gd, Nd, Ce) accumulated in the green alga Desmodesmus quadricauda but their intracellular localizations were distinctly different: lanthanum and gadolinium were localized in cytoplasm, while neodymium and cerium were in the chloroplast. The effect of lanthanum and neodymium, as representatives of these two groups, on growth, chlorophyll content and photosynthetic rate at different light intensities was studied. At the lowest light intensity used (50 µmol photons m
−2 s
−1), in the presence of lanthanides (Nd), growth was enhanced by as much as 36 % over lanthanide free control, and the photosynthetic rate increased by up to 300 %. At high light intensities (238, 460, and 750 µmol photons m
−2 s
−1), photosynthetic rate increased markedly, but there was no significant difference between rates in the presence or absence of lanthanides. However, growth, measured as a percentage of dry weight, if compared with lanthanide free control, increased at all light intensities (31, 39, and 20 %, respectively). The total amount of chlorophyll after lanthanide treatment increased by up to 21 % relative to the control culture, mainly due to an increase in the level of chlorophyll b. Addition of lanthanides caused a change in the chlorophyll a/b ratio from 4.583 in control cultivation, to 1.05. Possible mechanisms of lanthanide-induced photosynthetic change, alterations in photosynthetic structures, and increases in growth are discussed and compared with findings in higher plants. The hypothesis that the lanthanide effect could be due to formation of lanthanide-pheophytins was not confirmed as lanthanide pheophytins were not found in D. quadricauda. Furthermore, we have shown that the preferential incorporation of heavy isotopes of magnesium, namely
25Mg and
26Mg, into chlorophyll during photosynthesis that occurred in controls was diminished in the presence of lanthanides.
AB - Lanthanides (La, Gd, Nd, Ce) accumulated in the green alga Desmodesmus quadricauda but their intracellular localizations were distinctly different: lanthanum and gadolinium were localized in cytoplasm, while neodymium and cerium were in the chloroplast. The effect of lanthanum and neodymium, as representatives of these two groups, on growth, chlorophyll content and photosynthetic rate at different light intensities was studied. At the lowest light intensity used (50 µmol photons m
−2 s
−1), in the presence of lanthanides (Nd), growth was enhanced by as much as 36 % over lanthanide free control, and the photosynthetic rate increased by up to 300 %. At high light intensities (238, 460, and 750 µmol photons m
−2 s
−1), photosynthetic rate increased markedly, but there was no significant difference between rates in the presence or absence of lanthanides. However, growth, measured as a percentage of dry weight, if compared with lanthanide free control, increased at all light intensities (31, 39, and 20 %, respectively). The total amount of chlorophyll after lanthanide treatment increased by up to 21 % relative to the control culture, mainly due to an increase in the level of chlorophyll b. Addition of lanthanides caused a change in the chlorophyll a/b ratio from 4.583 in control cultivation, to 1.05. Possible mechanisms of lanthanide-induced photosynthetic change, alterations in photosynthetic structures, and increases in growth are discussed and compared with findings in higher plants. The hypothesis that the lanthanide effect could be due to formation of lanthanide-pheophytins was not confirmed as lanthanide pheophytins were not found in D. quadricauda. Furthermore, we have shown that the preferential incorporation of heavy isotopes of magnesium, namely
25Mg and
26Mg, into chlorophyll during photosynthesis that occurred in controls was diminished in the presence of lanthanides.
KW - chlorophyll
KW - lanthanide
KW - chemistry
KW - chloroplast
KW - drug effects
KW - green alga
KW - growth
KW - development and aging
KW - isolation and purification
KW - metabolism
KW - photosynthesis
KW - physiology
KW - Chlorophyll
KW - Chlorophyta
KW - Chloroplasts
KW - Lanthanoid Series Elements
KW - Photosynthesis
KW - Growth
KW - Green algae
KW - High-resolution electrospray mass spectrometry
KW - Photosynthetic rate
KW - Lanthanides
KW - Magnesium isotopes
UR - http://www.scopus.com/inward/record.url?scp=84964466669&partnerID=8YFLogxK
U2 - 10.1007/s11120-016-0263-9
DO - 10.1007/s11120-016-0263-9
M3 - Article
SN - 0166-8595
VL - 130
SP - 335
EP - 346
JO - Photosynthesis Research
JF - Photosynthesis Research
IS - 1-3
ER -