The salivary gland proteome of root-galling grape phylloxera (Daktulosphaira vitifoliae Fitch) feeding on Vitis spp.

M.W. Eitle, J.C. Carolan, M. Griesser, A. Forneck

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

The successful parasitisation of a plant by a phytophagous insect is dependent on the delivery of effector molecules into the host. Sedentary gall forming insects, such as grape phylloxera (Daktulosphaira vitifoliae FITCH, Phylloxeridae), secrete multiple effectors into host plant tissues that alter or modulate the cellular and molecular environment to the benefit of the insect. The identification and characterisation of effector proteins will provide insight into the host-phylloxera interaction specifically the gall-induction processes and potential mechanisms of plant resistance. Using proteomic mass spectrometry and in-silico secretory prediction, 420 putative effectors were determined from the salivary glands or the root-feeding D. vitifoliae larvae reared on Teleki 5C (V. berlandieri x V. riparia). Among them, 170 conserved effectors were shared between D. vitifoliae and fourteen phytophagous insect species. Quantitative RT-PCR analysis of five conserved effector candidates (protein disulfide-isomerase, peroxidoredoxin, peroxidase and a carboxypeptidase) revealed that their gene expression decreased, when larvae were starved for 24 h, supporting their assignment as effector molecules. The D. vitifoliae effectors identified here represent a functionally diverse group, comprising both conserved and unique proteins that provide new insight into the D. vitifoliae–Vitis spp. interaction and the potential mechanisms by which D. vitifoliae establishes the feeding site, suppresses plant defences and modulates nutrient uptake. © 2019 Eitle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
OriginalspracheEnglisch
Aufsatznummere0225881
Seiten (von - bis)e0225881
FachzeitschriftPLoS ONE
Jahrgang14
Ausgabenummer12
DOIs
PublikationsstatusVeröffentlicht - Dez. 2019
Extern publiziertJa

Forschungsfelder

  • Applied Viticulture & Oenology

IMC Forschungsschwerpunkte

  • Regional economics and agrobusiness

ÖFOS 2012 - Österreichischen Systematik der Wissenschaftszweige

  • 401117 Weinbau

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