Abstract
The yeast gene, GRC5 (growth control), is a member of the highly conserved QM gene family, the human member of which has been associated with the suppression of Wilms' tumor. GRC5 encodes ribosomal protein L10, which is thought to play a regulatory role in the translational control of gene expression. A revertant screen identified four spontaneous revertants of the mutant grc5-1(t)s allele. Genetic and phenotypic analysis showed that these represent one gene, NMD3, and that the interaction of NMD3 and GRC5 is gene-specific. NMD3 was previously identified as a component of the nonsense-mediated mRNA decay pathway. The point mutations within NMD3 reported here may define a domain important for the functional interaction of Grc5p and Nmd3p.
| Original language | English |
|---|---|
| Pages (from-to) | 419-429 |
| Number of pages | 11 |
| Journal | Current Genetics |
| Volume | 34 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
Keywords
- messenger RNA
- ribosome protein
- article
- controlled study
- gene expression
- gene mutation
- genetic analysis
- growth regulation
- human
- multigene family
- nephroblastoma
- nonhuman
- nonsense mutation
- phenotype
- point mutation
- priority journal
- protein interaction
- revertant
- saccharomyces cerevisiae
- translation regulation
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA Primers
- Gene Expression Regulation
- Genes
- Fungal
- Humans
- Molecular Sequence Data
- Mutation
- Phenotype
- Protein Biosynthesis
- Ribosomal Proteins
- Saccharomyces cerevisiae
- Sequence Homology
- Amino Acid
- Wilms Tumor
- Eukaryota
IMC Research Focuses
- Medical biotechnology
ÖFOS 2012 - Austrian Fields of Study
- 304005 Medical biotechnology
